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Klenow fragment radio labeling12/13/2023 The labeled probes are used in various hybridization techniques, such as Southern and Northern blots, in-situ hybridization and screening of gene libraries. Fragments can be labeled directly in low melting temperature agarose gel slices. The labeling mix method enables the labeling of small amounts of DNA (10-20ng), such as restriction fragments isolated from gels. This rapid labeling is accomplished with the use of the Klenow fragment, which lacks 5′-3′ exonuclease activity, and by the use of nonamer primers giving more efficient priming from the template at 37✬. The DNA labeling mix allows the labeling of the template DNA to a specific activity of 2x109dpm/3g after only 10 minutes of incubation. The reaction mixture contains random oligonucleotides, a Klenow fragment of DNA Polymerase I, dATP, dGTP, dTTP and a reaction buffer concentrate. The labeling mix system is a specially developed reaction mixture for enhanced convenience and performance. By substituting a radiolabeled nucleotide for a non-radioactive equivalent in the reaction mixture, the newly synthesized complementary DNA is made radioactive. The complementary DNA strand is synthesized by a “klenow” fragment of DNA Polymerase I, using the random oligonucleotides as primers. The AuPreP Random Primer Labeling Mix System is based on the hybridization of oligonucleotides of all possible sequences to the denatured template DNA to be labeled. The AuPreP Random Primer DNA Labeling Mix System is a specially designed premixed solution for the labeling of DNA with radiolabeled dCTP using random sequence oligonucleotides. Home > DNA/RNA Extraction DNA Sequencing Service > AuPreP TM DNA/RNA Kits>AuPreP™ random Primer labeling MixĪuPreP Random Primer DNA Labeling Mix System Vaccine & Bio Pharma : Drug development.JAX Mice: Gold Standard in Genetic Purity.Metabolic Disease Research Diabetes, Obesity, Cosmeticeuticals.Airocide - For Biosensitive Lab Environment, Sanitization.Phytodiagnostics Tools ELISA Kits Reagents Controls.Clones - cDNA, shRNA, miRNA clones Clones - cDNA, shR.PLANT - Tissue Culture & Molecular Biology Tools.ELISA Kits, Immunology & Cell Biology Products.Tissue Culture Media, Trypsin & its Substitutes.Life Technologies India: - FBS, Tissue Culture Media, Molecular Biology, Proteomics, Mycoplasma, Vaccine & Bioproduction, Elisa Kits
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